Integrin Alpha V Rabbit Polyclonal Antibody

• Catalog Number: orb10927
• Category: Antibodies
• Description: Integrin Alpha V Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC-Fr, IHC-P, IP, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Integrin Alpha V
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-1:2000, IHC-P=1:100-1:500, IHC-F=1:100-1:500, ICC/IF=1:50-1:200, IF=1:100-1:500, IP=1:20-1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 125 kDa
• Target: ITGAV
• UniProt ID: P06756
• RRID: AB_10751534
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: CD51; Integrin alpha five; integrin alpha V beta 3
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Burcu Biltekin 1, Ayhan Bilir, Ismail Seckin, Gozde Erkanli Senturk In vitro effects of heparin-binding epidermal growth factor on adhesion stage of implantation Rom J Morphol Embryol, 64(4), 493-500 (2023)

PubMed: 38184829

Applications

ICC, WB

Reactivity

Human

Shi, Kai et al. Clinicopathological and prognostic values of fibronectin and integrin αvβ3 expression in primary osteosarcoma World J Surg Oncol, 17, 23 (2019)

PubMed: 30691475

Blank control: U937 (blue). Primary Antibody: Rabbit Anti-Integrin Alpha V + Beta 3 (CD51+CD61) antibody (orb10927), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (orb10927, 1 µg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.

Cell: MCF-7, Concentration: 1:100, Host/Isotype: Rabbit/IgG, Flow cytometric analysis of Rabbit IgG isotype control (Cat#: orb10927) on MCF-7 (green) compared with control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L) secondary antibody.

Sample: HUVEC (human) cell Lysate at 40 ug, Primary: Anti-Integrin Alpha V + Beta 3 (CD51+CD61) (orb10927) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 116 kD, Observed band size: 120 kD.

Tissue/Cell: Human laryngeal tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Integrin Alpha V + Beta 3 (CD51 + CD61) Polyclonal Antibody, Unconjugated (orb10927) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: Human lung cancer tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Integrin Alpha V + Beta 3 (CD51 + CD61) Polyclonal Antibody, Unconjugated (orb10927) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Integrin Alpha V + Beta 3 (CD51+CD61) Polyclonal Antibody, Unconjugated (orb10927) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.