Integrin Alpha V Rabbit Polyclonal Antibody

SKU: orb10927

Description

Integrin Alpha V Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, IP, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, IP=1:20-50
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Integrin Alpha V
• Target: ITGAV
• Molecular Weight: 125 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

CD51; Integrin alpha five; Integrin alpha-V light chain; ITGAV; Msk8;Vitronectin receptor subunit alpha; VNRA; VTNR.

Blank control: U937 (blue). Primary Antibody: Rabbit Anti-Integrin Alpha V + Beta 3 (CD51+CD61) antibody (orb10927), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (orb10927, 1 µg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.

Cell: MCF-7, Concentration: 1:100, Host/Isotype: Rabbit/IgG, Flow cytometric analysis of Rabbit IgG isotype control (Cat#: orb10927) on MCF-7 (green) compared with control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L) secondary antibody.

Sample: HUVEC (human) cell Lysate at 40 ug, Primary: Anti-Integrin Alpha V + Beta 3 (CD51+CD61) (orb10927) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 116 kD, Observed band size: 120 kD.

Tissue/Cell: Human laryngeal tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Integrin Alpha V + Beta 3 (CD51 + CD61) Polyclonal Antibody, Unconjugated (orb10927) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: Human lung cancer tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Integrin Alpha V + Beta 3 (CD51 + CD61) Polyclonal Antibody, Unconjugated (orb10927) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Integrin Alpha V + Beta 3 (CD51+CD61) Polyclonal Antibody, Unconjugated (orb10927) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

ITGAV

UniProt

P06756

References

Burcu Biltekin 1, Ayhan Bilir, Ismail Seckin, Gozde Erkanli Senturk In vitro effects of heparin-binding epidermal growth factor on adhesion stage of implantation Rom J Morphol Embryol, 64(4), 493-500 (2023)

PubMed: 38184829

Applications

ICC, WB

Reactivity

Human

Shi, Kai et al. Clinicopathological and prognostic values of fibronectin and integrin αvβ3 expression in primary osteosarcoma World J Surg Oncol, 17, 23 (2019)

PubMed: 30691475