Integrin alpha V + beta 6 Antibody

• Catalog Number: orb2315367
• Category: Antibodies
• Description: Integrin alpha V + beta 6 Antibody
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: AvB6 53a.2
• Tested applications: ELISA, FC, IF, WB
• Reactivity: Human
• Isotype: IgG2a
• Immunogen: Mouse fibroblasts retrovirally infected with cDNA to human beta6; Cells expressed a mouse/human avb6 chimera.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Dilution range: ELISA (For coating use Ab at 1-5ug/ml, order Ab without BSA); Immunoprecipitation (1-2ug/500ug protein); ,Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (0.5-1ug/ml);,Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 150-160kDa under non-reducing conditions. Under reducing conditions|cleaved into 2 bands of ~125-130kDa +20-25kDa
• Target: ITGAV
• Entrez: 3685, 3694
• UniProt ID: P06756, P18564
• Expression System: Bioreactor
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: antigen identified by monoclonal antibody L230; CD
• Note: For research use only
• Application notes: Positive Control: Squamous cell carcinoma cells. A375M melanoma cell line. A549 cell line
• Expiration Date: 12 months from date of receipt.