Integrin alpha E Rabbit Polyclonal Antibody

SKU: orb6344

Description

Integrin alpha E Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1ug/test
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Integrin alpha-E heavy chain (1101-1179/1179aa)
• Target: ITGAE
• Molecular Weight: 110/128 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Itgae; A530055J10; alpha-E1; alpha-M290; aM290; CD103; MGC183601; antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide; ITAE_HUMAN; Integrin alpha-E; HML-1 antigen; Integrin alpha-IEL; Mucosal lymphocyte 1 antigen; Integrin alpha-E heavy chain; integrin alpha-E precursor.

Molt-4 cells were fixed with 4% PFA for 10 min at room temperature, permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD103 Antibody (orb6344) at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Sample: Lymph node (Mouse) Lysate at 40 ug, Primary: Anti-Integrin alpha E2 (orb6344) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 110/128 kD, Observed band size: 128 kD.

Sample: U937 (Human) Cell Lysate at 30 ug, Raji (Human) Cell Lysate at 30 ug, Primary: Anti-Integrin alpha E2 (orb6344) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 110/128 kD, Observed band size: 110 kD.

Quick Database Links

Gene Symbol

ITGAE

UniProt

P38570

References

Ayantika Kundu,Pratiti Ghosh, Biswadev Bishayi Vitexin along with verapamil downregulates efflux pump P-glycoprotein in macrophages and potentiate M1 to M2 switching via TLR4-NF-κB-TNFR7 pathway in Lipopolysaccharide treated mice Immunobiology, 229, 152767 (2024)

PubMed: 38103391

Applications

WB

Reactivity

Mouse

Rituparna Ghosh 1, Biswadev Bishayi Neutralization of TLR2 in combination with either TNF-α or IL-1β antibody reduces the severity of septic arthritis through STAT3/mTOR signalling in lymphocytes Cell Immunol, (2024)

PubMed: 39312873

Applications

WB

Reactivity

Mouse

Husseini, Mahmoud et al. Heme Oxygenase-1 Induction Prevents Autoimmune Diabetes in Association With Pancreatic Recruitment of M2-Like Macrophages, Mesenchymal Cells, and Fibrocytes Endocrinology, 156, 3937-3949 (2015)

PubMed: 26252059