Insulin Receptor Rabbit Polyclonal Antibody

SKU: orb10940

Description

Insulin Receptor Rabbit Polyclonal Antibody

Research Area

Cancer Research, Cardiovascular Research, Cell Biology, Diabetes Research, Insulin and Insulin Like, Insulin and Insulin-like Proteins, Metabolism, Receptor Tyrosne Kinases

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Rabbit, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Insulin Receptor (51-150/1384aa)
• Target: INSR
• Molecular Weight: 80/152 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

CD 220; CD220; CD220 antigen; HHF 5; HHF5; INSR; IR; INSR_HUMAN; Insulin receptor subunit alpha.

Blank control: Raji (blue). Primary Antibody: Rabbit Anti-Insulin Receptor alpha antibody (orb10940), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice. Primary antibody (orb10940, 1 µg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.

Sample: Lane 1: HepG2 (Human) Cell Lysate at 30 ug, Lane 2: 293T (Human) Cell Lysate at 30 ug, Lane 3: Lovo (Human) Cell Lysate at 30 ug, Lane 4: Molt-4 (Human) Cell Lysate at 30 ug, Lane 5: SW480 (Human) Cell Lysate at 30 ug, Lane 6: K562 (Human) Cell Lysate at 30 ug, Primary: Anti-Insulin Receptor (orb10940) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 120 kD, Observed band size: 120 kD.

Sample: Lane 1: MCF-7 (Human) Cell Lysate at 30 ug, Lane 2: A549 (Human) Cell Lysate at 30 ug, Lane 3: 293T (Human) Cell Lysate at 30 ug, Lane 4: Hela (Human) Cell Lysate at 30 ug, Lane 5: Liver (Rat) Lysate at 40 ug, Lane 6: Lymph node (Mouse) Lysate at 40 ug, Lane 7: MOLT-4 (Human) Cell Lysate at 30 ug, Lane 8: NIH/3T3 (Mouse) Cell Lysate at 30 ug. Primary: Anti-Insulin Receptor (orb10940) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 120 kD, Observed band size: 120 kD.

Tissue/Cell: rat liver tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Insulin Receptor/CD220 Polyclonal Antibody, Unconjugated (orb10940) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

INSR

UniProt

P06213