INPP5J Antibody

SKU: orb1256884

Description

INPP5J Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:10 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 60-360 of human INPP5J (NP_001002837.1).
• Target: INPP5J
• Molecular Weight: Observed: 107kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

INPP5J, PIB5PA, INPP5, PIPP

Western blot analysis of extracts of various cell lines, using INPP5J antibody (orb1256884) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat liver using INPP5J Antibody (orb1256884) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat kidney using INPP5J Antibody (orb1256884) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using INPP5J Antibody (orb1256884) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using INPP5J antibody (orb1256884). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

INPP5J

UniProt

Q15735