iNOS Rabbit Polyclonal Antibody (PE)

• Catalog Number: orb124697
• Category: Antibodies
• Description: iNOS Rabbit Polyclonal Antibody (PE)
• Target: iNOS
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: PE
• Reactivity: Rat
• Predicted Reactivity: Mouse, Rabbit
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from mouse NOS-2 (14-100/1144aa)
• Tested applications: IF
• Dilution range: IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Alternative names: i NOS; Nitric Oxide Synthase, Inducible; HEP NOS;
• Research Area: Cancer Research, Hypoxia
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: RAW264.7. Primary Antibody (green line): Rabbit Anti-iNOS antibody (orb124697), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.