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Inhibin beta B Rabbit Polyclonal Antibody

Catalog Number: orb13510

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb13510
CategoryAntibodies
DescriptionInhibin beta B Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IHC-Fr, IHC-P, WB
Predicted ReactivityBovine, Canine, Guinea pig, Porcine, Sheep
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human Inhibin beta B (251-350/407aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
MW13/45 kDa
TargetINHBB
UniProt IDP09529
RRIDAB_10750819
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesMGC157939; Activin A; Activin AB alpha polypeptide
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NoteFor research use only
Expiration Date12 months from date of receipt.
Inhibin beta B Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat ovary tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Inhibin beta B) Polyclonal Antibody, Unconjugated (orb13510) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Inhibin beta B Rabbit Polyclonal Antibody

Sample: A431 (Human) Cell Lysate at 40 ug, Primary: Anti-Inhibin beta B (orb13510) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45 kD, Observed band size: 50 kD.

Inhibin beta B Rabbit Polyclonal Antibody

Sample: Brain (Mouse) Lysate at 40 ug, Primary: Anti-Inhibin beta B (orb13510) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45 kD, Observed band size: 50 kD.

Inhibin beta B Rabbit Polyclonal Antibody

Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Mouse Breast tissue lysates, Lane 3: Mouse Placenta tissue lysates, Lane 4: Mouse Liver tissue lysates, Lane 5: Rat Testis tissue lysates, Lane 6: Rat Breast tissue lysates, Lane 7: Human A431 cell lysates, Lane 8: Human HepG2 cell lysates, Primary: Anti-Inhibin beta B (orb13510) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 13/45 kDa, Observed band size: 46 kDa.

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