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INDOL1 Antibody
SKU: orb20285
Description
Images & Validation
−Item 1 of 1
| Tested Applications | ELISA, WB |
|---|---|
| Dilution range | Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx. 50kDa band observed in Human Kidney, Liver, Placenta, Tonsil, Lung and Ileum lysates (calculated MW of 47.07kDa according to NP_919270.3). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature. |
| Reactivity | Human |
| Predicted Reactivity | Bovine, Canine |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Target | INDOL1 |
| Molecular Weight | 47.07 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Disclaimer | For research use only |
Alternative Names
−anti 3-dioxygenase 2 antibody, anti 3-dioxygenase-like protein 1 antibody, anti EC 1.13.11. antibody, anti I23O2_HUMAN antibody, anti INDOL 1 antibody, anti Indoleamine 2 antibody, anti Indoleamine 2,3 dioxygenase 2 antibody, anti Indoleamine 2,3 dioxygenase like 1 protein antibody, anti Indoleamine 2,3 dioxygenase like protein 1 antibody, anti Indoleamine pyrrole 2,3 dioxygenase like 1 antibody, anti Indoleamine pyrrole 2,3 dioxygenase like protein 1 antibody, anti Indoleamine-pyrrole 2 antibody
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Primary incubation 1 hour at room temperature. Images A, B, C: Human Kidney, Lung, Ileum lysate at primary Ab concentration 2 µg/ml, Images D, E, F: Human Placenta, Tonsil and Liver lysate at primary Ab concentration 3 µg/ml. (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
INDOL1 Antibody (orb20285)
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