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Catalog Number | orb2663259 |
---|---|
Category | Small Molecules |
Description | Biorbyt's Protein A Agarose to the surface of highly cross-linked agarose gel microspheres in a high density orientation. While natural Protein A contains five IgG-binding regions as well as regions of unknown function, recombinant Protein A reduces non-specific adsorption by removing the albumin- and cell surface-binding sites and retaining only five IgG-binding regions. Recombinant Protein A is better suited to bind mouse IgG2a, IgG2b, IgA, rabbit IgG, and human IgG1, IgG2, and IgG4.Biorbyt's Protein A Agarose are suitable for the isolation and purification of IgG from samples such as cell lysates, cell secretion supernatants, serum, ascites, and more. |
Purity | 98.00% |
Storage | -20°C |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
IP, WB | |
Sepharose | |
The product can be used for binding IgG from human, rabbit, goat, mouse and rat. It has strong binding of human, rabbit, cow, sheep, and goat polyclonal antibodies, mouse IgG2a, IgG2b, IgG3, and rat IgG2a. Protein A/G also has moderate affinity for mouse and rat IgG1 and IgG2c. It binds with weak affinity to rat IgG2b. Sepharose Protein A/G can be used for immunoprecipitation and purification of monoclonal antibodies. |