IgM Heavy Chain Antibody

SKU: orb248394

Description

This antibody recognizes a protein of 75kDa, identified as the mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. The antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution range: Flow cytometry: 0.5-1ug/million cells, Immunofluorescence: 0.5-1ug/ml, Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance. 1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b, kappa
• Clone No.: IM373
• Immunogen: Recombinant heavy chain of human IgM was used as the immunogen for this antibody.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC testing of human tonsil stained with IgM heavy chain antibody.

IHC testing of human tonsil stained with IgM heavy chain antibody.

Western blot testing of Raji cell lysate with IgM heavy chain antibody. Expected molecular weight: 70-75 kDa.

SDS-PAGE Analysis of Purified, BSA-Free IgM Heavy Chain Antibody (clone IM373). Confirmation of Integrity and Purity of the Antibody.