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Catalog Number | orb334311 |
---|---|
Category | Proteins |
Description | Recombinant Human Programmed Cell Death 1 Ligand 1 is produced by our Mammalian expression system and the target gene encoding Phe19-Thr239 is expressed with a mFc tag at the C-terminus. |
Tag | C-mFc |
Form/Appearance | Lyophilized from a 0.2 um filtered solution of PBS, pH 7.4. |
UniProt ID | Q9NZQ7 |
Expression System | Human Cells |
Biological Origin | Human |
Expression Region | Phe19-Thr239 |
Endotoxins | Less than 0.1 ng/ug (1 EU/ug) |
Storage | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
Alternative names | Programmed Cell Death 1 Ligand 1; PD-L1; PDCD1 Lig Read more... |
Note | For research use only |
Application notes | Mammalian expression system and the target gene encoding Phe19-Thr239 is expressed with a mFc tag at the C-terminus. |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 52.3 kDa after removal of the signal peptide. | |
Mammalian |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 35-42 kDa based on Tris-Bis PAGE result. |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 63-68 kDa based on Tris-Bis PAGE result. |
Greater than 95% as determined by SDS-PAGE. | |
52.7 kDa | |
Mammalian cell |
Unconjugated | |
98% | |
26.0 kDa | |
Human PD-L1, His Tag (orb257752) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # NP_054862.1 ). |