Human PD-L1 Protein, mFc-His tag

• Catalog Number: orb689384
• Category: Proteins
• Description: Recombinant human PD-L1 protein with C-terminal mouse Fc and 6×His tag
• Reactivity: Human
• Tag: C-Mouse Fc and 6×His Tag
• Form/Appearance: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
• MW: The protein has a predicted molecular mass of 52.3 kDa after removal of the signal peptide.
• Target: PDL1
• UniProt ID: Q9NZQ7
• Source: Mammalian
• Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
• Buffer/Preservatives: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
• Alternative names: PD-L1;CD274;B7-H1;PDCD1L1;PDCD1LG1
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

Human CD28 Protein, mFc-His Tag on SDS-PAGE under reducing condition.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human B7-1, hFc tagged protein orb689457 can bind Human CD28, mFc-His tagged protein (orb689375) in a linear range of 125-4000 ng/ml.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human B7-2, hFc tagged protein orb689458 can bind Human CD28, mFc-His tagged protein (orb689375) in a linear range of 62.5-4000 ng/ml.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28, mFc-His tagged protein (orb689375) 1 μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.