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Catalog Number | orb257752 |
---|---|
Category | Proteins |
Description | Human PD-L1, His Tag is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # NP_054862.1 ). |
Reactivity | Human |
Tag | C-6×His |
Form/Appearance | Powder |
Purity | 98% |
Conjugation | Unconjugated |
MW | 26.0 kDa |
Target | PD-L1, B7-H1 |
UniProt ID | Q9NZQ7 |
Protein Sequence | NP_054862.1 |
Source | Human PD-L1, His Tag (orb257752) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # NP_054862.1 ). |
Expression System | HEK293 |
Biological Origin | Human |
Expression Region | Phe 19 - Arg 238 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_054862.1 |
Storage | -20℃ |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | PD-L1,CD274,B7-H1,PDCD1L1,PDCD1LG1 Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.0 kDa. The protein migrates as 30-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Lai, Wei-Yun et al. A Novel PD-L1-targeting Antagonistic DNA Aptamer With Antitumor Effects Mol Ther Nucleic Acids, 5, e397 (2016)
SDS-PAGE analysis of Human PD-L1 protein
Line graph illustrates about the sensitivity range of Human PD-L1 protein using different concentrations
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 52.3 kDa after removal of the signal peptide. | |
Mammalian |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 26.0 kDa after removal of the signal peptide.The apparent molecular mass of PDL1-His is approximately 33-35 kDa due to glycosylation. | |
Mammalian |
Unconjugated | |
95% | |
27.1 kDa | |
Cynomolgus / Rhesus macaque PD-L1, His Tag (orb257750) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # G7PSE7-1). In the region Phe 19 - Arg 238, the AA sequence of Cynomolgus and Rhesus macaque PD-L1 are homologus. |
Unconjugated | |
95% | |
26.6 kDa | |
Mouse PD-L1, His Tag (orb257762) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Thr 238 (Accession # NP_068693). |
98.00% | |
28.48 kDa (predicted); 38.01 kDa (reducing conditions) |