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Human IL-1 beta Protein
Catalog Number: orb257615
| Catalog Number | orb257615 |
|---|---|
| Category | Proteins |
| Description | Human IL-1 beta, premium grade is expressed from E. coli cells. It contains AA Ala 117 - Ser 269 (Accession # P01584-1). |
| Target | IL-1 beta |
| Conjugation | Unconjugated |
| Tag | Native |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Purity | 95% |
| Protein Sequence | NP_000567.1 |
| UniProt ID | P01584 |
| MW | 17.5 kDa |
| Application notes | This protein carries no "tag". The protein has a calculated MW of 17.5 kDa. The protein migrates as 18 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE). |
| Expression System | E. coli |
| Endotoxins | 0.01 EU per μg |
| Biological Origin | Human |
| Biological Activity | Human IL-1 RII, Fc Tag (Cat. No. IL2-H4256) captured on CM5 chip via anti-human IgG Fc antibodies surface can bind Human IL-1 beta, premium grade (Cat. No. ) with an affinity constant of 0.622 nM as determined in a SPR assay (Biacore T200) (Routinely tested). |
| Expression Region | Ala 117 - Ser 269 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Note | For research use only |
| NCBI | NP_000567.1 |
SDS-PAGE analysis of Human IL-1 beta protein
Human IL-1 RII, Fc Tag (Cat. No. orb257592) captured on CM5 chip via anti-human IgG Fc antibodies surface can bind Human IL-1 beta, premium grade (Cat. No. orb257615) with an affinity constant of 0.622 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
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Human IL1B protein (Active) [orb358966]
> 97% as determined by SDS-PAGE and HPLC.
17.3 kDa
E.Coli
10 μg, 100 μg, 500 μg - Item 1 of 1
- Item 1 of 1
- Item 1 of 2
Human IL1B Protein, hFc Tag [orb1173751]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 43.5 kDa after removal of the signal peptide.The apparent molecular mass of IL1B-hFc is approximately 35-55 kDa due to glycosylation.
Mammalian
10 μg, 100 μg, 50 μg - Item 1 of 1
Human Caspase 1 protein [orb603953]
Greater than 90% as determined by SDS-PAGE.
43.8 kDa
E.coli
20 μg, 100 μg, 1 mg
