Human IL-1 beta Protein

• Catalog Number: orb257615
• Category: Proteins
• Description: Human IL-1 beta, premium grade is expressed from E. coli cells. It contains AA Ala 117 - Ser 269 (Accession # P01584-1).
• Reactivity: Human
• Tag: Native
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 17.5 kDa
• Target: IL-1 beta, IL-1F2
• UniProt ID: P01584
• Source: Human IL-1 beta, premium grade (orb257615) is expressed from E. coli cells. It contains AA Ala 117 - Ser 269 (Accession # P01584-1).
• Expression System: E. coli
• Biological Origin: Human
• Biological Activity: Human IL-1 RII, Fc Tag captured on CM5 chip via anti-human IgG Fc antibodies surface can bind Human IL-1 beta, premium grade with an affinity constant of 0.622 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
• Expression Region: Ala 117 - Ser 269
• Endotoxins: 0.01 EU per μg
• NCBI: NP_000567.1
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: IL1B,IL-1BETA,IL1F2,IL-1β
• Note: For research use only
• Application notes: This protein carries no "tag". The protein has a calculated MW of 17.5 kDa. The protein migrates as 18 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human IL-1 beta protein

Human IL-1 RII, Fc Tag (Cat. No. orb257592) captured on CM5 chip via anti-human IgG Fc antibodies surface can bind Human IL-1 beta, premium grade (Cat. No. orb257615) with an affinity constant of 0.622 nM as determined in a SPR assay (Biacore T200) (Routinely tested).