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Human IL-1 beta Protein, His Tag
Catalog Number: orb762423
| Catalog Number | orb762423 |
|---|---|
| Category | Proteins |
| Description | Human IL-1 beta, His Tag is expressed from E. coli cells. It contains AA Ala 117 - Ser 269 (Accession # P01584-1). |
| Target | IL-1 beta |
| Conjugation | Unconjugated |
| Tag | N-10×His |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Purity | 95% |
| Protein Sequence | NP_000567.1 |
| UniProt ID | P01584 |
| MW | 19.3 kDa |
| Application notes | This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 19.3 kDa. The protein migrates as 19-20 kDa under reducing (R) condition (SDS-PAGE). |
| Expression System | E. coli |
| Endotoxins | 1.0 EU per μg |
| Biological Origin | Human |
| Biological Activity | Human IL-1 RII, Fc Tag (Cat. No. IL2-H4256) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human IL-1 beta, His Tag (Cat. No. ) with an affinity constant of 0.571 nM as determined in a SPR assay (Biacore 8K) (Routinely tested). |
| Expression Region | Ala 117 - Ser 269 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Note | For research use only |
| NCBI | NP_000567.1 |
Human IL-1 RII, Fc Tag (Cat. No. orb257592) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human IL-1 beta, His Tag (Cat. No. orb762423) with an affinity constant of 0.571 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Human IL1B Protein, His Tag [orb2701853]
> 95%, determined by SDS-PAGE
This protein contains the mature form of human IL1β (NP_000567.1) (Ala 117-Ser 269) was expressed.
100 μg, 20 μgHuman IL1B Protein, His Tag [orb2701854]
> 95%, determined by SDS-PAGE
This protein contains the pro form of human IL1β (NP_0005671) (Met 1-Ser 269) was fused with a polyhistidine tag at the N-terminus and expressed from E. coli.
20 μg, 5 μgRecombinant Human IL-1 beta Protein, His Tag [orb1551467]
> 95% by SDS-PAGE.
Recombinant Human IL-1 beta Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ala117-Ser269) of human IL-1 beta (Accession #NP_000567.1) fused with an initial Met at the N-terminus and a 6��His Tag at the C-terminus.
200 μg, 100 μgIL-1 beta/IL-1F2 Protein, Human, Recombinant (His) [orb1957665]
93.80%
32.3 kDa (predicted); 34 kDa (reducing conditions)
500 μg, 100 μg, 200 μgIL-1 beta/IL-1F2 Protein, Human, Recombinant (His & Avi), Biotinylated [orb1960717]
98.00%
20.1 kDa (predicted) same as Tris-Bis PAGE result.
500 μg, 100 μg, 1 mg
