Cytokeratin 6 antibody

SKU: orb2276480

Description

Cytokeratin 6 antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

• Tested Applications: IHC
• Dilution range: 1:100-1:200
• Reactivity: Human
• Application Notes:
  Positive Control: Tonsil: A strong CK6 staining should be seen in all epithelial cells of the surface epithelium except the basal cell layer where staining should be absent or weak. Negative Control: Tonsil: CK6 staining should be absent in lymphocytes and all other mesenchymal cells. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: MSVA-606R
• Immunogen: Recombinant fragment of human KRT6 (exact sequence is proprietary)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

CK6A, CK6B, CK6C, CK6D, CK6E, Keratin Type II Cytoskeletal 6A, Keratin Type II Cytoskeletal 6B, Keratin Type II Cytoskeletal 6C, Keratin Type II Cytoskeletal 6D, Keratin Type II Cytoskeletal 6E, KRT6, KRT6A, KRT6B, KRT6C, KRT6D, KRT6E

Colorectal adenocarcinoma with strong KRT6 positivity in about 50% of tumor cells.

Squamous cell carcinoma of the uterine cervix with strong Cytokeratin 6 positivity.

Ectocervix - Cervical squamous epithelium with strong cytokeratin 6 immunostaining of all cell layers except basal cells.

Quick Database Links

UniProt

P48668, P02538, P04259