CCNB1 Antibody

SKU: orb1252007

Description

CCNB1 Antibody

Research Area

Cancer Biology, Cell Biology

Images & Validation

• Tested Applications: IF, IHC-P
• Reactivity: Human, Mouse
• Application Notes:
  Immunofluorescence: 1-2 µg/mLImmunohistochemistry (FFPE): 1-2 µg/mL for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Titering of the Cyclin B1 antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: BCLB1-1
• Immunogen: Recombinant human full-length protein was used as the immunogen for the Cyclin B1 antibody.
• Target: CCNB1
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Disclaimer: For research use only

Alternative Names

G2/mitotic-specific cyclin-B1, CCNB1, CCNB

IHC testing of FFPE human ovarian carcinoma with Cyclin B1 antibody (clone BCLB1-1).

IHC testing of FFPE human prostate carcinoma with Cyclin B1 antibody (clone BCLB1-1).

IHC testing of FFPE human tonsil with Cyclin B1 antibody (clone BCLB1-1).

Quick Database Links

Gene Symbol

CCNB1

UniProt

P14635