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Item 1 of 4
Item 1 of 4
IMPDH2 monoclonal antibody (M01), clone 1E12-B6
Catalog Number: orb2292716
| Catalog Number | orb2292716 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody raised against a full length recombinant IMPDH2. |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Clone Number | 1E12-B6 |
| Tested applications | ELISA, IP, WB |
| Reactivity | Human |
| Isotype | IgG1 kappa |
| Immunogen | IMPDH2 (AAH12840, 1 a.a. ~ 514 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Conjugation | Unconjugated |
| Protein Sequence | MADYLISGGTSYVPDDGLTAQQLFNCGDGLTYNDFLILPGYIDFTADQVDLTSALTKKITLKTPLVSSPMDTVTEAGMAIAMALTGGIGFIHHNCTPEFQANEVRKVKKYEQGFITDPVVLSPKDRVRDVFEAKARHGFCGIPITDTGRMGSRLVGIISSRDIDFLKEEEHDCFLEEIMTKREDLVVAPAGITLKEANEILQRSKKGKLPIVNEDDELVAIIARTDLKKNRDYPLASKDAKKQLLCGAAIGTHEDDKYRLDLLAQAGVDVVVLDSSQGNSIFQINMIKYIKDKYPNLQVIGGNVVTAAQAKNLIDAGVDALRVGMGSGSICITQEVLACGRPQATAVYKVSEYARRFGVPVIADGGIQNVGHIAKALALGASTVMMGSLLAATTEAPGEYFFSDGIRLKKYRGMGSLDAMDKHLSSQNRYFSEADKIKVAQGVSGAVQDKGSIHKFVPYLIAGIQHSCQDIGAKSLTQVRAMMYSGELKFEKRTSSAQVEGGVHSLHSYEKRLF |
| NCBI | AAH12840 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | In 1x PBS, pH 7.4 |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Detection limit for recombinant GST tagged IMPDH2 is approximately 0.3 ng/ml as a capture antibody.
Immunoprecipitation of IMPDH2 transfected lysate using anti-IMPDH2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IMPDH2 MaxPab rabbit polyclonal antibody.
Western Blot analysis of IMPDH2 expression in transfected 293T cell line by IMPDH2 monoclonal antibody (M01), clone 1E12-B6. Lane 1: IMPDH2 transfected lysate (55.8 KDa). Lane 2: Non-transfected lysate.
Western blot analysis of IMPDH2 over-expressed 293 cell line, cotransfected with IMPDH2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with IMPDH2 monoclonal antibody (M01), clone 1E12-B6 (Cat # orb2292716). GAPDH (36.1 kDa) used as specificity and loading control.
