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Catalog Number | orb1526228 |
---|---|
Category | Antibodies |
Description | IMPACT Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IP, WB |
Reactivity | Human |
Immunogen | between 270 and 320 |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | IMPACT |
UniProt ID | Q9P2X3 |
NCBI | NP_060909.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | RWD domain containing 5; RWDD5; protein IMPACT; im Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human IMPACT by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-IMPACT antibody orb1526228 used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. IMPACT was also immunoprecipitated by rabbit anti-IMPACT antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
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