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Product Overview
Product Name Beta Lactoglobulin antibody
Catalog Number orb21203
Species/HostRabbit
ReactivityBovine
ConjugationUnconjugated
Tested applicationsDOT, IEP, IP
Immunogen Purified intact bovine beta-lactoglobulin was used. The resulting polyclonal antiserum contains Ab's against several epitopes on the beta-lactoglobulin molecule.
Target Beta Lactoglobulin. The defined antibody specificity is directed to beta-lactoglobulin as tested against bovine milk. In immunoelectrophoresis and double radial immunodiffusion (Ouchterlony) no reaction has been obtained with bovine serum.
Product Properties
Form/Appearance Vial with 1 ml lyophilized antiserum.
Concentration Total protein and IgG concentrations in the antiserum are comparable to those in pooled rabbit serum. No foreign proteins added.
Preservatives No preservative added.
Storage The lyophilised antiserum is shipped at ambient temperature and may be stored at 4°C; prolonged storage at or below -20°C. Reconstitute the lyophilised antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at 4°C, not refrozen, and preferably used the same day.
Note For research use only.
ClonalityPolyclonal
Adsorption Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Product Description

Precipitating polyclonal rabbit antiserum to bovine beta-lactoglobulin. Beta-lactoglobulin is the dominant protein in bovine milk, synthesized in mammary gland tissue. It is a glycoprotein present mainly as a dimer at intermediate concentrations near the isoelectric point. The molecular weight of the monomeric subunit is near 17,000. It is very sensitive to denaturation. Any treatment of native milk and also prolonged storage in the deepfreeze will lead first to polymerization (octamers and higher polymers) aggregation and finally to denaturation. Three genetic variants A, B and C have been identified. They differ very little in the amino acid constellation an their behavior under pH and temperature various conditions. No immunological differences was found between the variants. Beta-lactoglobulin can be purified in crystallized form. Allergic reactions of infants to bovine milk proteins are well known. The actual process by which such reactions develop and their impact on the infant's health have not been unambiguously established. Circulating antibodies to beta-lactoglobulin have been demonstrated in sera of some infants. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Notes
Application Notes Homologous proteins of different species frequently share antigenic determinants. Cross-reactivity has been observed with milk of sheep and goat.

IE: In immunoelectrophoresis use 2 μl serum, plasma or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ulantiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. In single radial immunodiffusion and electroimmunodiffusion use 0.5 to 1.0 percent antiserum in the agar gel.
Validation Images
Immunoelectrophoresis shows beta Lactoglobulin antibody containing antiserum reaction with cow milk and purified beta lactoglobulin but not with serum
Immunoelectrophoresis shows beta Lactoglobulin antibody containing antiserum reaction with cow milk and purified beta lactoglobulin but not with serum
Ouchterlony immunodiffusion showing beta Lactoglobulin antibody containing antiserum strong reaction with an antigen in bovine milk but not with serum
Ouchterlony immunodiffusion showing beta Lactoglobulin antibody containing antiserum strong reaction with an antigen in bovine milk but not with serum
Immunoprecipitation of Beta Lactoglobulin antibody containing antiserum with bovine milk
Immunoprecipitation of Beta Lactoglobulin antibody containing antiserum with bovine milk
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