ILVBL Rabbit Polyclonal Antibody

SKU: orb3015

Description

ILVBL Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=3ug/Test
• Reactivity: Human
• Predicted Reactivity: Canine, Equine, Mouse, Porcine, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ILVBL (301-400/632aa)
• Target: ILVBL
• Molecular Weight: 68 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Acetolactate synthase homolog; Acetolactate synthase like protein; Acetolactate synthase-like protein; AHAS; ILV2H; 209L8; IlvB(bacterial acetolactate synthase) like; IlvB like protein; IlvB-like protein; Ilvbl; ILVBL_HUMAN.

Sample: HepG2 Cell (Human) Lysate at 30 ug, Primary: Anti-ILVBL (orb3015) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 68kD, Observed band size: 68kD.

Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-ILVBL antibody (orb3015), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Hcclm3 (Human) Cell Lysate at 30 ug, MCF-7 (Human) Cell Lysate at 30 ug, A431 (Human) Cell Lysate at 30 ug, Primary: Anti-ILVBL (orb3015) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 68 kD, Observed band size: 68 kD.

Quick Database Links

Gene Symbol

ILVBL

UniProt

A1L0T0