ILK-1 Rabbit Polyclonal Antibody

• Catalog Number: orb10918
• Category: Antibodies
• Description: ILK-1 Rabbit Polyclonal Antibody
• Target: ILK
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Gallus
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human ILK-1 (301-400/452aa)
• UniProt ID: Q13418
• RRID: AB_10750649
• MW: 50 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: 59 kDa serine/threonine protein kinase; 59 kDa ser
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (mouse heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ILK-1) Polyclonal Antibody, Unconjugated (orb10918) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ILK-1) Polyclonal Antibody, Unconjugated (orb10918) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Heart tissue lysates, Lane 2: Mouse Muscle tissue lysates, Lane 3: Rat Heart tissue lysates, Lane 4: Rat Muscle tissue lysates, Lane 5: Human MCF-7 cell lysates, Lane 6: Human Huvec cell lysates, Lane 7: Human U87MG cell lysates, Primary: Anti-ILK-1 (orb10918) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kDa, Observed band size: 50 kDa.

Sample: Lung (Mouse) Lysate at 40 ug, Heart (Mouse) Lysate at 40 ug, Primary: Anti-ILK-1 (orb10918) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 50 kD.