IL7R Rabbit Polyclonal Antibody

• Catalog Number: orb10917
• Category: Antibodies
• Description: IL7R Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human IL-7Ra (251-350/459aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 60-90 kDa
• Target: IL7R
• UniProt ID: P16871
• RRID: AB_10752479
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: IL-7Rα; IL-7Ra; Interleukin-7 receptor subunit alp
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: U937 (blue). Primary Antibody: Rabbit Anti-IL-7Ra antibody (orb10917), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (orb10917, 1 µg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.

Sample: Lane 1: Mouse Spleen tissue lysates, Lane 2: Mouse Thymus tissue lysates, Lane 3: Mouse Lymph node tissue lysates, Lane 4: Mouse Lung tissue lysates, Lane 5: Rat Spleen tissue lysates, Lane 6: Rat Thymus tissue lysates, Lane 7: Human K562 cell lysates, Lane 8: Human U-2 OS cell lysates, Lane 9: Human U-87 MG cell lysates, Primary: Anti-IL-7Ra (orb10917) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 70 kD.