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Catalog Number | orb1272267 |
---|---|
Category | Antibodies |
Description | IL7 Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | NYR-hi7 |
Tested applications | ELISA, NeA, WB |
Reactivity | Human |
Immunogen | The monoclonal antibody was produced in BALB/c x C57BL/6 F1 mice using recombinant hIL-7 as the immunizing antigen. |
Concentration | batch dependent |
Dilution range | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL 7 (0.5 ng/mL), a concentration of 0.05-0.08 μg/mL of this antibody is required. ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human IL-7 when used with our biotinylated antigen affinity purified anti-human IL-7 (XP-5197Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.Western Blot:To detect hIL-7 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-7 is 2.0-4.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
Target | IL7 |
UniProt ID | P13232 |
NCBI | P13232 |
Storage | IL-7 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted. |
Alternative names | Interleukin 7Interleukin-7IL-7, IL-7 Read more... |
Note | For research use only |
Application notes | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL 7 (0.5 ng/mL), a concentration of 0.05-0.08 μg/mL of this antibody is required. ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human IL-7 when used with our biotinylated antigen affinity purified anti-human IL-7 (XP-5197Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.Western Blot:To detect hIL-7 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-7 is 2.0-4.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions. |
Expiration Date | 12 months from date of receipt. |
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