Il7 Antibody

• Catalog Number: orb1272440
• Category: Antibodies
• Description: Il7 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, NeA, WB
• Reactivity: Mouse
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-7 (murine IL-7).
• Concentration: batch dependent
• Dilution range: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mIL-7 (0.60 ng/mL), a concentration of 0.012 - 0.02 μg/mL of this antibody is required. ELISA:To detect mIL-7 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-7. Sandwich:To detect mIL-7 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine IL-7as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-7.Western Blot:To detect mIL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-7 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• Target: Il7
• UniProt ID: P10168
• NCBI: P10168
• Storage: IL-7 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
• Alternative names: Il-7, hlb368, A630026I06Rik, Il-7, Interleukin-7IL
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

To detect Murine IL-7 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Murine IL-7 (orb1272439) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine IL-7.

To detect Murine IL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine IL-7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

To detect Murine IL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine IL-7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.