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    IL6ST antibody

    Catalog Number: orb393081

    DispatchUsually dispatched within 5-10 working days
    $ 337.00
    Catalog Numberorb393081
    CategoryAntibodies
    DescriptionRabbit polyclonal antibody to IL6ST.
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsIF, IH, WB
    ReactivityHuman, Mouse, Porcine, Primate, Rat
    ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CD130. The exact sequence is proprietary.
    Dilution rangeWB: 1:500:1000
    Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    ConjugationUnconjugated
    TargetIL6ST
    Entrez16195, 3572
    UniProt IDP40189, Q00560, P40190
    SourceRabbit
    StorageShipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
    Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    Alternative namesInterleukin-6 receptor subunit beta; IL-6 receptor
    Read more...
    NoteFor research use only
    Expiration Date12 months from date of receipt.
    IL6ST antibody

    Western blot analysis of CD130 expression in HCT116 (A), AML12 (B) whole cell lysates. (Predicted band size: 103 kD; Observed band size: 130 kD)

    IL6ST antibody

    Immunohistochemical analysis of CD130 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    IL6ST antibody

    Immunofluorescent analysis of CD130 staining in LO2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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