IL6ST Antibody

• Catalog Number: orb1270375
• Category: Antibodies
• Description: IL6ST Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This CD130 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 871-899 amino acids from the C-terminal region of human CD130.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:2000For IHC-P starting dilution is: 1:25
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 104 kDa
• Target: IL6ST
• UniProt ID: P40189
• NCBI: P40189
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Interleukin-6 receptor subunit beta, IL-6 receptor
• Note: For research use only
• Application notes: For WB starting dilution is: 1:2000For IHC-P starting dilution is: 1:25
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: MDA-MB-231 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: SW480 whole cell lysate Lane 5: A549 whole cell lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: HT-1080 whole cell lysate Lysates/proteins at 20 ug per lane.

Antibody staining CD130 in Human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: MDA-MB-231 whole cell lysate Lane 3: SW480 whole cell lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:2000 dilution + SW480 whole cell lysates Lysates/proteins at 20 ug per lane.

Western blot analysis in MDA-MB231 cell line lysates (35 ug/lane).