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Catalog Number | orb1274475 |
---|---|
Category | Antibodies |
Description | IL6ST Antibody, KO Validated |
Target | IL6ST |
Clonality | Polyclonal |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
Form/Appearance | Liquid |
Concentration | batch dependent |
Buffer/Preservatives | Liquid |
Purification | Affinity purification |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 678-857 of human IL6ST (NP_001177910.1). |
UniProt ID | P40189 |
MW | Observed: 130kDa |
Tested applications | WB |
Application notes | WB: 1:500 - 1:2000 |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | Interleukin-6 receptor subunit beta, IL-6 receptor Read more... |
Note | For research use only |
Western blot analysis of extracts from normal (control) and IL6ST knockout (KO) A-549 cells, using IL6ST antibody (orb1274475) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.