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IL6ST Antibody, KO Validated

Catalog Number: orb1274475

DispatchUsually dispatched within 5-10 working days
$ 800.00
Catalog Numberorb1274475
CategoryAntibodies
DescriptionIL6ST Antibody, KO Validated
TargetIL6ST
ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Form/AppearanceLiquid
Concentrationbatch dependent
Buffer/PreservativesLiquid
PurificationAffinity purification
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 678-857 of human IL6ST (NP_001177910.1).
UniProt IDP40189
MWObserved: 130kDa
Tested applicationsWB
Application notesWB: 1:500 - 1:2000
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesInterleukin-6 receptor subunit beta, IL-6 receptor
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NoteFor research use only
IL6ST Antibody, KO Validated

Western blot analysis of extracts from normal (control) and IL6ST knockout (KO) A-549 cells, using IL6ST antibody (orb1274475) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.