You have no items in your shopping cart.
Cart summary
Item 1 of 1
IL6 antibody
Catalog Number: orb345214
| Catalog Number | orb345214 |
|---|---|
| Category | Antibodies |
| Description | IL6 antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ELISA, FC, IHC, WB |
| Reactivity | Mouse |
| Isotype | IgG |
| Immunogen | Anti-IL-6 is an IgG fraction antibody prepared from rabbit antiserum after repeated immunizations with recombinant mouse IL-6 protein produced in E.coli. |
| Concentration | 1.0 mg/mL |
| Dilution range | ELISA: 1:10,000, IHC: User Optimized, WB: 1:1000 |
| Form/Appearance | Lyophilized |
| Purity | This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for mouse IL-6 protein. A BLAST analysis was used to suggest cross-reactivity with IL-6 from mouse sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-6 from other sources has not been determined. |
| Conjugation | Unconjugated |
| UniProt ID | P08505 |
| NCBI | NP_112445.1 |
| Storage | Store anti-IL-6 at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Buffer/Preservatives | None |
| Alternative names | rabbit anti-IL-6 antibody, rabbit anti-Interleukin Read more... |
| Note | For research use only |
| Application notes | This purified antibody has been tested for use in Immunohistochemistry and western blotting and is suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 21.7 kDa in size corresponding to the mature 187 amino acid mouse IL-6 protein by western blotting in appropriate cell lysate or extract. |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of transferred to nitrocellulose using IL6 antibody
- Item 1 of 17
- Item 1 of 3
Human IL-6 R alpha / CD126 Protein [orb257625]
Unconjugated
95%
39.4 kDa
Human IL-6 R alpha, His Tag (orb257625) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Pro 365 (Accession # NP_000556.1).
1 mg, 50 μg - Item 1 of 7
- Item 1 of 5
- Item 1 of 4
Submit a review
Filter by Rating
- 5 stars
- 4 stars
- 3 stars
- 2 stars
- 1 stars