IL6 Antibody

• Catalog Number: orb1272268
• Category: Antibodies
• Description: IL6 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1.2-2B11-2G10
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: The monoclonal antibody was produced in BALB/c x C57BL/6 F1 mice using recombinant hIL-6 as the immunizing antigen.
• Concentration: batch dependent
• Dilution range: ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant human IL-6 when used with our biotinylated antigen affinity purified anti-human IL-6 (XP-5193Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.Western blot:To detect hIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-6 is 0.25-0.50 ng/lane, under reducing or non-reducing conditions.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• Target: IL6
• UniProt ID: P05231
• NCBI: P05231
• Storage: IL-6 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.
• Alternative names: Interleukin 6, IFNB2, Interleukin-6, B-cell stimul
• Note: For research use only
• Application notes: ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant human IL-6 when used with our biotinylated antigen affinity purified anti-human IL-6 (XP-5193Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.Western blot:To detect hIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-6 is 0.25-0.50 ng/lane, under reducing or non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

To detect hIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-6 is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.

To detect hIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-6 is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.

This antibody stained formalin-fixed, paraffin-embedded sections of human cervical squamous cell carcinoma. The recommended concentration is 0.125 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.