IL32 Antibody

• Catalog Number: orb1239659
• Category: Antibodies
• Description: IL32 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Anti-IL-32 antibody (orb1239659) was raised against a peptide corresponding to 16 amino acids near the center of human IL-32. The immunogen is located within amino acids 50 - 100 of IL-32.
• Concentration: 1 mg/mL
• Dilution range: WB: 0.5-5 μg/mL.Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 26kDObserved: 26 kD
• Target: IL32
• UniProt ID: P24001
• NCBI: AAH09401
• Storage: IL-32 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: IL-32 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: IL-32 Antibody: NK4, TAIF, TAIFa, TAIFb, TAIFc, TA
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation with Recombinant Protein. Loading: 30 ng of human IL-32 recombinant protein per lane. Antibodies: IL-32, orb1239659 (Lane 1: 0.5 µg/mL, Lane 2: 1 µg/mL, Lane 3: 2 µg/mL and Lane 4: 4 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 17kD.

Western Blot Validation in Jurkat Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: IL-32, orb1239659 (A: 2.5 µg/mL and B: 5 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.