IL32 Antibody

• Catalog Number: orb1239494
• Category: Antibodies
• Description: IL32 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Anti-IL-32 antibody (orb1239494) was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human IL-32. The immunogen is located within the last 50 amino acids of IL-32.
• Concentration: 1 mg/mL
• Dilution range: WB: 0.5-4 μg/mL; IHC: 2-10 μg/mL; IF: 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples; Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 26kDObserved: 26 kD
• Target: IL32
• UniProt ID: P24001
• NCBI: AAH09401
• Storage: IL-32 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: IL-32 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: IL-32 Antibody: NK4, TAIF, TAIFa, TAIFb, TAIFc, TA
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation with Recombinant Protein. Loading: 30 ng of human IL-32 recombinant protein per lane. Antibodies: IL-32, orb1239494 (Lane 1: 0.5 µg/mL, Lane 2: 1 µg/mL, Lane 3: 2 µg/mL and Lane 4: 4 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 17kD.

Western Blot Validation in Human Spleen Lysate, showing two isoforms of IL-32. Loading: 15 µg of lysates per lane. Antibodies: IL-32, orb1239494 (lane 1: 2 µg/mL and lane 2: 4 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry Validation of IL-32 in Human Spleen Tissue. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL-32 antibody (orb1239494) at 10 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of IL-32 in Human Spleen Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen cells labeling IL-32 with orb1239494 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Immunohistochemistry Validation of IL-32 in Mouse Spleen Tissue. Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-IL-32 antibody (orb1239494) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.