IL22BP Rabbit Polyclonal Antibody

• Catalog Number: orb6197
• Category: Antibodies
• Description: IL22BP Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Human, Rat
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human IL-22BP (165-263/263aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 28(hu)/25(mo, ra kDa
• Target: IL22RA2
• UniProt ID: Q969J5
• RRID: AB_10936368
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: IL22RA2; Interleukin-22 receptor subunit alpha-2;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: mouse spleen sells (blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA, Primary Antibody, dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA (green).

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IL22BP) Polyclonal Antibody, Unconjugated (orb6197) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.