IL21R Antibody

• Catalog Number: orb1238905
• Category: Antibodies
• Description: IL21R Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: IL-21 receptor antibody was raised against a synthetic peptide corresponding to amino acids 97 to 111 of human IL-21 receptor precursor.The immunogen is located within amino acids 80 - 130 of IL-21 Receptor.
• Concentration: 1 mg/ml
• Dilution range: IL-21 receptor antibody can be used for detection of IL-21 receptor by Western blot at 1-2 μg/mL. An approximately 60 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in human and rat samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 60 kDa Observed: 62 kDa
• Target: IL21R
• UniProt ID: Q9HBE5
• NCBI: NP_068570
• Storage: IL-21 Receptor antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: IL-21 Receptor Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: IL-21 Receptor Antibody: NILR, CD360, NILR, UNQ312
• Note: For research use only
• Application notes: IL-21 receptor antibody can be used for detection of IL-21 receptor by Western blot at 1-2 μg/mL. An approximately 60 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in human and rat samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of IL-21 receptor expression in human HepG2 cell lysate with IL-21 receptor antibody at (A) 1 and (B) 2 µg/ml.

Immunohistochemistry of IL-21 receptor in rat lung with IL-21 receptor antibody at 10 µg/mL.

Immunofluorescence of IL-21 Receptor in Rat Lung cells with IL-21 Receptor antibody at 20 µg/mL.

Immunofluorescence of IL21R in A431 cells with IL 21 Receptor antibody at 20 µg/mL. Green: IL-21 Receptor Antibody (orb1238905) Blue: DAPI staining.