IL20 Rabbit Polyclonal Antibody

• Catalog Number: orb13501
• Category: Antibodies
• Description: IL20 Rabbit Polyclonal Antibody
• Target: IL20
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Rat
• Predicted Reactivity: Human, Mouse
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from mouse IL-20 (51-150/176aa)
• UniProt ID: Q9JKV9
• RRID: AB_10753435
• MW: 17 kDa
• Tested applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Interleukin-20; four alpha helix cytokine; IL10D;
• Research Area: Cell Biology, Interleukins
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Le Roux, Mélina et al. IL-20 Cytokines Are Involved in Epithelial Lesions Associated with Virus-Induced COPD Exacerbation in Mice Biomedicines, 9, 1838 (2021)

PubMed: 34944654

Paraformaldehyde-fixed, paraffin embedded (rat skin tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IL-20) Polyclonal Antibody, Unconjugated (orb13501) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.