IL2 Antibody

• Catalog Number: orb1259059
• Category: Antibodies
• Description: IL2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant protein of human IL2
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 18kDa
• Target: IL2
• UniProt ID: P60568
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: Interleukin 2Interleukin-2, T-cell growth factor,
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using IL2 antibody (orb1259059) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Western blot analysis of extracts of Raw264.7 cells, using IL2 antibody (orb1259059) at 1:1000 dilution. Raw264.7 cells were treated by LPS for 6 hours and Brefeldin A for 3 hours of stimulation. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded human tonsil using IL2 antibody (orb1259059) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung using IL2 antibody (orb1259059) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using IL2 antibody (orb1259059) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse spleen using IL2 antibody (orb1259059) at dilution of 1:100 (40x lens).