IL17E Rabbit Polyclonal Antibody

SKU: orb221442

Description

IL17E Rabbit Polyclonal Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Equine, Rabbit, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human IL17E (431-434/644aa)
• Target: IL25
• Molecular Weight: 17 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IL17E; IL-17e; IL-25; IL25_HUMAN; IL25; Interleukin-17E (IL-17E);

Protein: spleen (mouse) lysate at 30 ug, Primary: rabbit Anti-IL17E (orb221442) at 1:300, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000, Predicted band size: 17 kD, Observed band size: 17 kD.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-IL17E Polyclonal Antibody, Unconjugated (orb221442) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

IL25

UniProt

Q9H293