Il17a Antibody

• Catalog Number: orb1671703
• Category: Antibodies
• Description: Il17a Antibody
• Clonality: Recombinant
• Clone Number: MM17F3
• Tested applications: Blocking, ELISA
• Reactivity: Mouse
• Isotype: IgG kappa
• Immunogen: This antibody was raised by immunising C57Bl/6 mice with IL-17A cross-linked to ovalbumin to generate a highly immunogenic complex.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: Il17a
• UniProt ID: Q62386
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Interleukin-17A, IL17A, Cytotoxic T-lymphocyte-ass
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the Anti-IL-17A antibody MM17F3. Mouse splenocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA- permeusing 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control - black line) or the r version of MM17F3 (orb1671703 - blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.