IL17A Antibody

• Catalog Number: orb1239477
• Category: Antibodies
• Description: IL17A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Bovine, Porcine, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Anti-IL-17 antibody (orb1239477) was raised against a peptide corresponding to 16 amino acids near the center of mature human IL-17. The immunogen is located within amino acids 50-100 of IL-17.
• Concentration: 1 mg/mL
• Dilution range: WB: 0.125-2 μg/mL; IF: 5-20 μg/mL; IHC: 1-5 μg/mL .Antibody validated: Western Blot in human samples; Immunofluorescence in mouse samples; Immunohistochemistry in human and mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 17.5kDObserved: 18 kDa
• Target: IL17A
• UniProt ID: Q16552
• NCBI: NP_002181
• Storage: IL-17 antibody can be stored at 4°C for up to one year. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: IL-17 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: IL-17 Antibody: IL17, CTLA8, IL-17, IL-17A, IL17,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation with Recombinant Protein. Loading: 30 ng of human IL-17 recombinant protein per lane. Antibodies: IL-17 orb1239477, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.125 µg/mL, Lane 2: 0.25 µg/mL, Lane 3: 0.5 µg/mL.

Western Blot Validation with Human Spleen. Loading: 10 µg of lysates per lane. Antibodies: IL-17 orb1239477, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Exposure: 1 min. Lane 1: 1 µg/mL Lane 2: 2 µg/mL.

Immunohistochemistry Validation of IL-17 in Human Lymph Node. Immunohistochemical analysis of paraffin-embedded human lymph node tissue using anti-IL-17 antibody (orb1239477) at 1 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of IL-17 in Mouse Heart Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse heart issue labeling IL-17 with orb1239477 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunofluorescence Validation of IL-17 in Mouse A-20 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse A-20 Cells labeling IL-17 with orb1239477 at 5 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunofluorescence Validation of IL-17 in Mouse Thymus Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus tissue labeling IL-17 with orb1239477 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Immunohistochemistry Validation of IL-17 in Mouse Colon Tissue. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-IL-17 antibody (orb1239477) at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of IL-17 in Human Tonsil Tissue. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IL-17 antibody (orb1239477) at 5 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.