IL17A Antibody

• Catalog Number: orb1239678
• Category: Antibodies
• Description: IL17A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: IL-17 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human IL-17A.The immunogen is located within amino acids 40 - 90 of IL-17.
• Concentration: 1 mg/mL
• Dilution range: WB: 0.125-4 μg/mL.Antibody validated: Western Blot in human and mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 17.5kDObserved: 20 kD (Post-modification: 1 N-linked glycosylation)
• Target: IL17A
• UniProt ID: Q16552
• NCBI: NP_002181
• Storage: IL-17 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: IL-17 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: IL-17 Antibody: IL17, CTLA8, IL-17, IL-17A, IL17,
• Note: For research use only
• Application notes: WB: 0.125-4 μg/mL.Antibody validated: Western Blot in human and mouse samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western Blot Validation in Human THP-1 Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: IL-17 orb1239678 (A: 1 µg/mL and B: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in A-20 Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: IL-17 orb1239678 (A: 2 µg/mL and B: 4 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Recombinant Protein Test. Loading: 30 ng of human IL-17 recombinant protein per lane. Antibodies: IL-17 orb1239678, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.125 µg/mL, Lane 2: 0.25 µg/mL, Lane 3: 0.5 µg/mL.