IL13RA2 Rabbit Polyclonal Antibody

SKU: orb612204

Description

IL13RA2 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from mouse IL-13Ra2 (21-120/383aa)
• Target: IL13RA2
• Molecular Weight: 41 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Interleukin-13 receptor subunit alpha-2; IL-13 receptor subunit alpha-2; IL-13 receptor subunit alpha-2; subunit alpha-2; IL-13R-alpha-2; IL-13RA2; CD213a2; IL13R; IL-13R; IL13 receptor; IL13BP; Interleukin 13 binding protein; Interleukin-13-binding protein; I13R2_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IL-13Ra2) Polyclonal Antibody, Unconjugated (orb612204) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human U251 cell lysates, Lane 2: Human HeLa cell lysates, Primary: Anti-IL13RA2 (orb612204) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 41 kDa, Observed band size: 46 kDa.

Quick Database Links

Gene Symbol

IL13RA2