IL12B Rabbit Polyclonal Antibody

• Catalog Number: orb184291
• Category: Antibodies
• Description: IL12B Rabbit Polyclonal Antibody
• Target: IL-12B
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Equine, Porcine, Rat, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human IL12B (201-300/328aa)
• MW: 35 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CLMF; CLMF p40; CLMF2; Cytotoxic lymphocyte matura
• Research Area: Cell Biology, Inflammation, Interleukins, Macropha
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Protein: spleen (mouse) lysates at 40 ug, Primary: Anti-Il-12 beta (orb184291) at 1:300, Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1:5000, ECL excitated the fluorescence, Predicted band size: 35 kD, Observed band size: 37 kD.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-IL-12 beta Polyclonal Antibody, Unconjugated (orb184291) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.