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IL12_2 Antibody
Catalog Number: orb1270664
| Catalog Number | orb1270664 |
|---|---|
| Category | Antibodies |
| Description | IL12_2 Antibody |
| Clonality | Polyclonal |
| Tested applications | FC, IHC-P, WB |
| Reactivity | Human |
| Isotype | Rabbit Ig |
| Immunogen | This IL12_2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 756-783 amino acids from the C-terminal region of human IL12_2. |
| Antibody Type | Primary Antibody |
| Concentration | batch dependent |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | 97 kDa |
| Target | IL12RB2 |
| UniProt ID | Q99665 |
| NCBI | Q99665 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
| Alternative names | Interleukin-12 receptor subunit beta-2, IL-12 rece Read more... |
| Note | For research use only |
| Application notes | For FACS starting dilution is: 1:25For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50 |
| Expiration Date | 12 months from date of receipt. |
Overlay histogram showing A431 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37oC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed (OH191631) at 1/200 dilution for 40 min at 37oC. Isotype control antibody (blue line) was rabbit IgG (1 ug/1x10^6 cells) used under the same conditions. Acquisition of >10000 events was performed.
Western Blot at 1:2000 dilution Lane 1: THP-1 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in MDA-MB435 cell line lysates (35 ug/lane).
IL12RB2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsils tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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