IL-8/CXCL8 Rabbit Polyclonal Antibody

• Catalog Number: orb2563137
• Category: Antibodies
• Description: IL-8/CXCL8 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant human IL-8/CXCL8 protein
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 11 kDa
• Target: CXCL8
• UniProt ID: P10145
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Interleukin-8; Interleukin 8; Interleukin8; (Ala-I
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

20 ng human IL-8/CXCL8 protein-His protein per lane probed with IL-8/CXCL8 polyclonal antibody respectively, unconjugated (orb2563137) at 1:1000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.

U-87MG (H) cells were treated with or without Thapsigargin (1µM, 24h), 25 µg total protein per lane of cell lysates probed with IL-8/CXCL8 polyclonal antibody, unconjugated (orb2563137) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.