IL-6 Antibody

• Catalog Number: orb1272447
• Category: Antibodies
• Description: IL-6 Antibody
• Target: Il6
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Mouse
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Purification: Anti-mIL-6 specific antibody was purified by affinity chromatography employing immobilized mIL-6 matrix.
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-6 (murine Interleukin-6).
• UniProt ID: P08505
• Tested applications: ELISA, NeA, WB
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Il-6, Il-6, Interleukin-6, B-cell hybridoma growth
• Research Area: Immunology & Inflammation, Neuroscience
• Note: For research use only
• NCBI: P08505

To detect Murine IL-6 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Murine IL-6 (orb1272446) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine IL-6.

To detect Murine IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine IL-6 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

To detect Murine IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine IL-6 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained colchicine injected mouse brain (cingulate cortex) tissue. The primary antibody was incubated at 0.5 ug/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary.