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IL 4 Protein

IL 4 Protein

Catalog Number: orb429410

DispatchUsually dispatched within 5-10 working days
$ 250.00
Catalog Numberorb429410
CategoryProteins
DescriptionRecombinant of IL 4 protein
Form/AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
PurityGreater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Solubility (25°C)It is recommended to reconstitute the lyophilized Interleukin 4 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Protein SequenceHGCNDSPLR EIINTLNQVT EKGTPCTEMF VPDVLTATRN TTENELICRA SRVLRKFYFP RDVPPCLKNK SGVLGELRKL CRGVSGLNSL RSCTVNESTL TTLKDFLESL KSILRGKYLQ SCTSMS
SourceEscherichia Coli
Biological ActivityThe ED50 as determined in a cell proliferation assay using rat splenocytes is less than 2ng/ml corresponding to a specific activity of 500,000IU/mg.
StorageStability: Lyophilized Interleukin-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL4 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles
Buffer/PreservativesLyophilized from a 0.2µm filtered concentrated solution in PBS pH 7.4 and 5% trehalose.
Alternative namesBCGF, BCDF, B cell stimulating factor, BSF-1, Lymp
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NoteFor research use only
Application notesProtein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.2 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-4 as a Reference Standard
Expiration Date6 months from date of receipt.
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