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Catalog Number | orb1962176 |
---|---|
Category | Proteins |
Description | IL-4 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 18 KDa and the accession number is P05112. |
Tag | C-6xHis |
Purity | SDS-PAGE: 100%; SEC-HPLC: 99% |
MW | 18 KDa (reducing condition) |
UniProt ID | P05112 |
Protein Sequence | His25-Ser153 |
Expression System | HEK293 Cells |
Biological Origin | Human |
Biological Activity | IL-4 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 18 KDa and the accession number is P05112. |
Expression Region | His25-Ser153 |
Storage | -20°C |
Note | For research use only |
Application notes | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 15.8 kDa after removal of the signal peptide. The apparent molecular mass of IL4-His is approximately 15-25 kDa due to glycosylation. | |
E.coli |
> 95% as determined by SDS-PAGE | |
21 kDa |
> 90% as determined by SDS-PAGE | |
35-50 kDa |
SDS-PAGE: > 99%; SEC-HPLC: 98.4% | |
35-45 KDa (reducing condition) |
98.00% | |
17.8 kDa (predicted). Due to glycosylation, the protein migrates to 23-25 kDa based on Tris-Bis PAGE result. |