IL-27 Antibody

• Catalog Number: orb1238893
• Category: Antibodies
• Description: IL-27 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Anti-IL-27 antibody (orb1238893) was raised against a peptide corresponding to 16 amino acids near the amino terminus of human IL-27. The immunogen is located within the first 50 amino acids of IL-27.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 28 kDa Observed: 28 kDa
• Target: IL27
• UniProt ID: Q8NEV9
• NCBI: NP_663634
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: IL-27 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: IL-27 Antibody: p28, IL30, IL-27, IL27A, IL-27A, I
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation in Mouse A20 Cells. Loading: 15 µg of lysates per lane. Antibodies: IL-27 orb1238893, (A: 1 µg/mL, B: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: IL-27 orb1238893 (4 µg/mL), IL-27 orb1239663 (4 µg/mL), and beta-actin (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Recombinant Protein Validation. Loading: 30 ng of human recombinant IL-17 (IL-17A+IL-17B) protein per lane. Antibodies: IL-27 orb1238893, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 µg/mL, Lane 2: 1 µg/mL, Lane 3: 2 µg/mL.

Immunocytochemistry Validation of IL-27 in Mouse EL4 Cells. Immunocytochemical analysis of Mouse EL4 cells using anti-IL-27 antibody (orb1238893) at 10 µg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.