IL-23 Rabbit Polyclonal Antibody

• Catalog Number: orb10907
• Category: Antibodies
• Description: IL-23 Rabbit Polyclonal Antibody
• Target: Il23a
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human IL-23
• UniProt ID: Q9NPF7
• RRID: AB_10750981
• MW: 21 kDa
• Tested applications: ELISA, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Interleukin-23 subunit alpha; Il23a; IL 23; IL 23A
• Research Area: Cell Biology, Interleukins
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

A Wojtkowiak-Giera 1, D Kosik-Bogacka 2, N Łanocha-Arendarczyk 3, A Kolasa 4, K Kot 2, P Solarczyk 1, M Derda Toll-like receptors and inflammatory cytokines in the skin of Acanthamoeba spp. infected immunocompetent and immunosuppressed mice Exp Parasitol ., (2025)

PubMed: 40274043

Applications

IHC

Reactivity

Mouse

Sample: Lane 1: Mouse Ear tissue lysates, Lane 2: Human A431 cell lysates, Lane 3: Human Jurkat cell lysates, Primary: Anti-IL-23 (orb10907) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 21 kDa, Observed band size: 21 kDa.

Tissue/Cell: human rhinopolypus tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-IL-23 Polyclonal Antibody, Unconjugated (orb10907) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.