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Catalog Number | orb1959099 |
---|---|
Category | Proteins |
Description | IL-23 Protein, Human, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 57.3 kDa and the accession number is Q9NPF7&P43432. |
Tag | C-His |
Purity | 98.00% |
UniProt ID | P43432, Q9NPF7 |
MW | 57.3 kDa (predicted) |
Application notes | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Expression System | HEK293 Cells |
Biological Origin | Human,Mouse |
Biological Activity | IL-23 Protein, Human, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 57.3 kDa and the accession number is Q9NPF7&P43432. |
Expression Region | A DNA sequence encoding the human IL23A (Q9NPF7) (Met1-Pro189) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the mouse IL12B (P43432) (Met1-Ser335) was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human IL23A & the mouse IL12B heterodimer was purified. Predicted N terminal: Arg 20 & Met 23 |
Storage | -20°C |
Note | For research use only |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 50-60 (Mouse IL12 Beta) & 22 ( Human IL23 Alpha) kDa based on Tris-Bis PAGE result. |