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IL-23 Protein, Human, Mouse, Recombinant (His)

IL-23 Protein, Human, Mouse, Recombinant (His)

Catalog Number: orb1959099

DispatchUsually dispatched within 5-10 working days
$ 570.00
Catalog Numberorb1959099
CategoryProteins
DescriptionIL-23 Protein, Human, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 57.3 kDa and the accession number is Q9NPF7&P43432.
TagC-His
Purity98.00%
UniProt IDP43432, Q9NPF7
MW57.3 kDa (predicted)
Application notesA Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Expression SystemHEK293 Cells
Biological OriginHuman,Mouse
Biological ActivityIL-23 Protein, Human, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 57.3 kDa and the accession number is Q9NPF7&P43432.
Expression RegionA DNA sequence encoding the human IL23A (Q9NPF7) (Met1-Pro189) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the mouse IL12B (P43432) (Met1-Ser335) was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human IL23A & the mouse IL12B heterodimer was purified. Predicted N terminal: Arg 20 & Met 23
Storage-20°C
NoteFor research use only
  • Recombinant human IL-23 Alpha & mouse IL-12 Beta protein, C-His-Avi (HEK293) [orb1516411]

    > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC

    Due to glycosylation, the protein migrates to 50-60 (Mouse IL12 Beta) & 22 ( Human IL23 Alpha) kDa based on Tris-Bis PAGE result.

    20 μg